Tag Archives | Mutations

Isolation and Sequencing of Mutations by DHPLC | Genetics | Biology

In this article we will discuss about the isolation and sequencing of heteroduplex fragments from DHPLC analysis of somatic, low-frequency mutations. Introduction to Isolation and Sequencing of Mutations by DHPLC: Identification of somatic mutations in samples from solid tumors repre­sent some additional challenges compared to the identification of heterozy­gous germline mutations. Biopsies from solid tumors often contain various amounts of [...]

By |2017-03-15T04:10:02+00:00March 15, 2017|Genetics|Comments Off on Isolation and Sequencing of Mutations by DHPLC | Genetics | Biology
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Types of Mutagens: Radiation and Chemical | Genetics

Two types of mutagens are considered here which are mostly affected the humans producing different mutations resulting in a number of abnormalities: 1. Radiation Exposure 2. Chemical Mutagenesis. Type # 1. Radiation Exposure: High energy radiation or ionising radia­tion produces a genetic alteration or mutation at a very low dose also. It has been shown experimentally that a low dose [...]

By |2016-12-12T07:34:22+00:00December 12, 2016|Mutations|Comments Off on Types of Mutagens: Radiation and Chemical | Genetics
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DNA Repair Mechanisms Studied in Eukaryotes | Genetics

In this article we will discuss about the five main DNA repair mechanisms that are studied in eukaryotes. The mechanisms are: 1. Photo-Reactivation or Photo-Repair 2. Dark or Excision Repair 3. Nucleotide Excision Repair 4. Mismatch Repair 5. Post-Replication Repair. Mechanism # 1. Photo-Reactivation or Photo-Repair: This mechanism was discovered when bacteria that had been heavily irradiated with UV rays [...]

By |2016-12-12T07:24:35+00:00December 12, 2016|Eukaryotes|Comments Off on DNA Repair Mechanisms Studied in Eukaryotes | Genetics
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