In this article we will discuss about the isolation of pigments and their characterization. Theory of Plant Pigments: Plant pigments are mostly the colouring substances found either in cell sap (as water-soluble forms, or in the plastids (water-insoluble forms). Normally, the plastidial pigments are responsible for photosynthesis while cell sap pigments are responsible for coloration of floral parts and various [...]
The following points highlight the top four techniques used for the isolation of microbial mutants. The techniques are: 1. Direct Observation 2. Enrichment Technique 3. Replica-Plating Technique 4. The Ames Test. 1. Direct Observation Technique: In some cases, a colony growing on an agar plate can easily be seen to be different from the normal parental type (wild-type). For example, [...]
The below mentioned article provides a quick notes on Isolation of Protoplasts and Protoplast Culture. Klerker (1892) made unsuccessful attempts to isolate protoplasts. In 1909 Kunster isolated protoplasts by mechanical method. Effective technique of protoplast isolation was evolved by Cocking ('60), Nagata and Takebe ('70) and Bhojwani and Cocking ('72). Enzymes used for isolation of protoplasts are cellulase, hemicellulase and [...]