In this article we will discuss about:- 1. Meaning of PCR 2. Steps of PCR 3. Applications of PCR. Meaning of PCR: In genetic engineering, requirement of desirable amount of target DNA is indispensable to study genetic structure, function and gene manipulation. It is novel cell free techniques to obtain innumerable number of copies. This direct enzymatic amplification process is [...]
Exploitation of plant viruses as transformation vectors by massive infection may be harmful and even deleterious to the target plants. It is still however able to express and produce foreign proteins. Plant viruses must exhibit some of the exemplary features before they are considered as vectors. They should extend their broader host-range, spread of seed transmission and carry additional copies [...]
This article provides a case study on transgenic rice for stress tolerance. Production of agronomically useful transgenic rice has been given top priority in the improvement of crop plants. The loss of rice yield due to stress impact is estimated to cost at least several billion dollars a year. Genetic engineering offers effective way to deal with these problems. Moreover, [...]