A genomic library contains all the sequences present in the genome of an organism. In the construction of genomic libraries it is feasible to use vectors that could accommodate large size of inserts. The first step in the construction of genomic library is the isolation of genomic DNA, and entire DNA is subjected to restriction digestion. The fragmented DNA of [...]
In this article we will discuss about the process of T-DNA transfer. Once plant exudates activating expression of vir genes and Agrobacterium attach to the plant cells, the T-DNA region in the Ti plasmid undergo excision process to transfer into the plant cells. Excision process involves active participation of vir genes such as virD, virC and virE. Separation of single [...]
Gene transfer in cereals is becoming established at the backdrop of recombinant DNA technology. Until recently, gene transfer in plants was accomplished only through certain conventional methods like sexual hybridization. The entire scenario of transformation was changed after the advancement of genetic engineering. Utility of Agrobacterium tumeifaciens as biological vector was found to be successful among dicots. However, its potential [...]