In this article we will discuss about the methods of gene transfer. Gene transfer can be done by two methods: (i) Indirect Method through Vectors or Carriers and (ii) Direct or Vectorless Transfer Method. The first method includes: (a) Plasmids and Viruses and (b) Natural Genetic Engineer, (a) Plasmids and Viruses: Recombinant DNA technology involves the transfer of desired genes [...]
Genetic engineering is considered as a kind of biotechnology. This is a process in which the alteration of the genetic make-up of cells is done by deliberate and artificial means. This process involves transfer or replacement of genes to create recombinant DNA. This can be done by cutting DNA molecules at specific sites to get fragments containing desirable and useful [...]
In this article we will discuss about the steps and applications of Recombinant DNA Technology. Steps Involved in Recombinant DNA Technology: The sequences of steps in r-DNA technology are as follows: (i) Cell culture of cells with the required DNA sequences is obtained through tissue culture technique, (ii) Specific restriction endonuclease is selected from the specific bacterium, (iii) Restriction endonuclease [...]