The below mentioned article provides a study note on gene sequencing. When a particular gene of interest or a DNA fragment is isolated, the sequencing of that piece of DNA becomes essential. The sequence of DNA refers to the order of nucleotide bases along its sugar phosphate backbone. No technique can determine the sequence of bases in an entire gene [...]
In this article we will discuss about the plant genome project. The major revolution in the study of genomes of different species was brought about due to the availability of recombinant DNA and PCR technologies. These techniques helped in preparation of molecular maps of many plant and animal genomes. The objective of genomic research in any species is to sequence [...]
The below mentioned article provides a note on Polymerase Chain Reaction (PCR). Gene Amplification: Polymerase Chain Reaction (PCR): PCR provides a simple and ingenious method for exponential amplification of specific DNA sequences by in vitro DNA synthesis, i.e., this technique has made it possible to synthesize large quantities of DNA fragments without cloning it. Kary Mulis in 1985 developed the [...]