The below mentioned article provides a study note on gene sequencing. When a particular gene of interest or a DNA fragment is isolated, the sequencing of that piece of DNA becomes essential. The sequence of DNA refers to the order of nucleotide bases along its sugar phosphate backbone. No technique can determine the sequence of bases in an entire gene [...]
In this article we will discuss about the plant genome project. The major revolution in the study of genomes of different species was brought about due to the availability of recombinant DNA and PCR technologies. These techniques helped in preparation of molecular maps of many plant and animal genomes. The objecÂtive of genomic research in any species is to sequence [...]
The below mentioned article provides a study note on molecular mapping. Preparation of linkage map based on recombination data is always handicapped due to non-availability of mutants for many genes. This limitation has largely been overcome in recent years by molecular mapping through ISH (in situ hybridization), Restriction mapping, RFLP (Restriction Fragment Length Polymorphism) analysis, RAPD (Random Amplified Polymorphic DNA) [...]