In this article we will discuss about the purpose and basic steps of genetic engineering. Purpose of Genetic Engineering: The interest in genetic engineering principally is due to its varied applications (Fig. 22.1): 1. Production of varieties of plants having particular desirable characteristics (e.g., resistance or tolerance to disease, drought, 'development of CMS line, etc.). 2. Improvement in the production [...]
The following points highlight the two main types of screening techniques. The types are: 1. Colony Hybridisation Technique 2. Blotting Techniques. Type # 1. Colony Hybridisation Technique: This technique has been developed by Gaustein and Hogness (1975). It requires the radioactively labelled DNA probe with a sequence complementary to at-least one part of the desired DNA. The probe may be [...]
In this article we will discuss about:- 1. Isolation of DNA to be Cloned 2. Insertion of Foreign DNA Fragment into a Vector 3A. Transfer of Recombinant DNA into Bacterial Cell 3B. Cloning in Eukaryotes 4. Detection of Recombinant Clone. Gene Cloning Technique # 1. Isolation of DNA to be Cloned: The DNA of interest, i.e., target DNA may [...]