In this article we will discuss about the purpose and basic steps of genetic engineering. Purpose of Genetic Engineering: The interest in genetic engineering principally is due to its varied applications (Fig. 22.1): 1. Production of varieties of plants having particular desirable characteristics (e.g., resistance or tolerance to disease, drought, 'development of CMS line, etc.). 2. Improvement in the production [...]
The following points highlight the two main types of screening techniques. The types are: 1. Colony Hybridisation Technique 2. Blotting Techniques. Type # 1. Colony Hybridisation Technique: This technique has been developed by Gaustein and Hogness (1975). It requires the radioactively labelled DNA probe with a sequence complementary to at-least one part of the desired DNA. The probe may be [...]
In this article we will discuss about the plant genome project. The major revolution in the study of genomes of different species was brought about due to the availability of recombinant DNA and PCR technologies. These techniques helped in preparation of molecular maps of many plant and animal genomes. The objective of genomic research in any species is to sequence [...]