In this article we will discuss about the meaning and advantages of pollen culture. Meaning of Pollen Culture: Microspore or the immature pollen can be used as the explant to get the haploid plants directly. For pollen or microspore culture the flower buds are collected, surface sterilized and the anther lobes are dissected out from the flower buds as before. [...]
The following points highlight the five main enzymes involved in genetic engineering. The enzymes are: 1. Restriction Endonuclease 2. DNA Ligase 3. Alkaline Phosphatase 4. DNA Polymerase and the Klenow Fragment 5. Reverse Transcriptase. Genetic engineering became possible with the discovery of mainly two types of enzymes: the cutting enzymes called restriction endonucleases and the joining enzymes called ligases. Restriction [...]
In this article we will discuss about vectors used in genetic engineering. Cloning Vector: By cloning, one can produce unlimited amounts of any particular fragment of DNA. In principle, the DNA isolated and cut pieces are introduced into a suitable host cell, usually a bacterium such as Escherichia coli, where it is replicated, as the cell grows and divides. However, [...]