In this article we will discuss about the meaning and advantages of pollen culture. Meaning of Pollen Culture: Microspore or the immature pollen can be used as the explant to get the haploid plants directly. For pollen or microspore culture the flower buds are collected, surface sterilized and the anther lobes are dissected out from the flower buds as before. [...]
The below mentioned article highlight the top two methods for culturing isolated pollen. Method 1: This method is described here for the culture of isolated pollen of tobacco. This technique can be considered as the basic protocol for pollen culture and involves the following steps: Step 1. Selection of suitable unopened flower bud, sterilization, excision of anther without filament are [...]
The below mentioned article provides the advantages of pollen culture over anther culture. Anther culture is an efficient way for the production of haploids from the microspores present within the intact anther. In this process, there is always the possibility that somatic cells of the anther that are diploid, will also respond to the culture condition and so produce unwanted [...]