The below mentioned article provides a note on Polymerase Chain Reaction (PCR). Gene Amplification: Polymerase Chain Reaction (PCR): PCR provides a simple and ingenious method for exponential amplification of specific DNA sequences by in vitro DNA synthesis, i.e., this technique has made it possible to synthesize large quantities of DNA fragments without cloning it. Kary Mulis in 1985 developed the [...]
In this article we will discuss about the genomic and cDNA libraries. Genomic DNA Library: A genomic library is a collection of independently isolated vector linked DNA fragments derived from a single organism. It contains at least one copy of every DNA sequence in the genome. An ideal library is one that represents all of the sequences with smallest possible [...]
The below mentioned article provides a study note on molecular mapping. Preparation of linkage map based on recombination data is always handicapped due to non-availability of mutants for many genes. This limitation has largely been overcome in recent years by molecular mapping through ISH (in situ hybridization), Restriction mapping, RFLP (Restriction Fragment Length Polymorphism) analysis, RAPD (Random Amplified Polymorphic DNA) [...]