The BIS means Bureau of Indian Standards. The BIS norms are also called ‘Standards for Bio fertilizers’ or ‘Standards for Existence of Bio fertilizers’, which are given below:-
1. Standards for Rhizobium (RI):
IS 8268:2001
(i) Viable Cell Count: 108 Viable Rhizobium Cells/g of the Carrier within 15 day s of manufacture and 107 Cells/g of Carrier within 15 days before the Expiry at Storage temperature 25-30°C
(ii) pH: 6-7.5
(iii) Moisture Percentage: 35-40
(iv) Shelf Life: 6 Months
(v) Carrier:
Peat, Lignite, Peat Soil, Humus or Similar Material Neutralized with CaCO3 and then sterilized. Carrier should be in the form of powder capable of passing through 150 to 212 Micron (72-100 Mesh) IS Sieve
(vi) Marking on the Packet:
The Inoculatent Packet shall have the following information:
(a) Name of the Product
(b) Name and Address of the Manufacturer
(c) Carrier Used
(d) Batch Number
(e) Date of Manufacture
(f) Date of Expiry
(g) Net Mass
(h) Crop for which intended
(i) Storage Instructions
(j) Application: RI shall show Effective Nodulation Formation. The Total Dry Mass of Inoculated Control and at Least 50% more than the Controlled one
2. Standards for Azotobacter (AI):
IS 9138:2002
(i) Viable Cell Count: 10 Viable Azotobacter Cells/g of the Carrier within 15 Days of Manufacture and 106 Cells/g of Carrier within 15 Days before the Expiry at Storage Temperature 25-30°C
(ii) pH: 6.5-7
(iii) Moisture Percentage: 35-40
(iv) Shelf Life: 6 Months
(v) Carrier: Peat, Lignite, Peat Soil, Humus or similar material neutralized with CaCO3 and then Sterilized. Carrier should be in the form of powder capable of passing through 150 to 212 Micron (72- 100 Mesh) IS Sieve.
(vi) Notrogen Fixation: The Minimum Nitrogen Fixed Shall not be Less than 100 mg Per g of Sucrose used
(vii) Germination Test: AI shall have a good effect on seed germination
(viii) Marking on the Pocket:
The Inoculant Packet shall have the following information:
(a) Name of the Product
(b) Name and address of the Manufacturer
(c) Carrier used
(d) Batch number
(e) Date of manufacture
(f) Date of expiry
(g) Net Mass
(h) Crop for which Intended
(i) Storage Instructions
(j) Application Instructions
3. Inoculant (AI):
IS: 14806:2000
Azospirillum (ASP)
(i) Viable Cell Count: 107 Viable Azospirillum Cells/g of the Carrier within 15 days of manufacture and 106 Cells/g of Carrier within 15 days before the Expiry at Storage Temperature 25-30°C
(ii) pH: 7-8
(iii) Moisture Percentage: 35-40
(iv) Shelf Life: 6 Months
(v) Carrier: Peat, Lignite, Peat Soil, Humus or Similar Material Neutralized with CaC03 and then Sterilized. Carrier should be in the form of Powder Capable of Passing through 150 to 212 Microns (72-100 Mesh) IS Sieve.
(vi) Plant Test: ASP Shall Show Effective Root Development on all Crops against which the Inoculant is intended to be used. There should be 10% increase in Plants over Dry Mass or Uninoculated Control
(vii) Marking on the Packet:
The Inoculants Packet shall have the following information:
(a) Name of the Product
(b) Name and address of the Manufacture
(c) Carrier Used
(d) Batch Number
(e) Date of Manufacture
(f) Date of Expiry
(g) Net Mass
(h) Crop for which Intended
(i) Storage Instructions
(j) Application Instructions.
4. Phosphate Solubilizing Bacterial Inoculants (PSB):
IS-14807:2000
(i) Viable Cell Count: 107 Viable Bacterial Cells/g of the Carrier within 15 days of Manufacture and 106 Cells/g of carrier within 15 Days before the Expiry at Storage Temperature 25-30°C
(ii) pH: 6.5-7.5
(iii) Moisture Percentage: 35-40
(iv) Shelf Life: 6 Months
(v) Carrier: Peat, Lignite, Peat Soil, Humus or Similar Material Neutralized with CaCO3 and then Sterilized. Carrier should be in the form of Powder Capable of Passing through 150 to 212 Micro (72- 100 Mesh) IS Sieve.