The below mentioned article highlight the top two methods for culturing isolated pollen. Method 1: This method is described here for the culture of isolated pollen of tobacco. This technique can be considered as the basic protocol for pollen culture and involves the following steps: Step 1. Selection of suitable unopened flower bud, sterilization, excision of anther without filament are [...]
Let us make an in-depth study of the meaning and principle of anther and pollen culture. Meaning of Anther and Pollen Culture: What is Anther Culture? Anther culture is a technique by which the developing anthers at a precise and critical stage are excised aseptically from unopened flower bud and are cultured on a nutrient medium where the microspores within [...]
The following points highlight the top seven steps involved in anther culture. Step 1. Collect the flower buds of Nicotiana tabacum at the onset of flowering (normally 3- 4 months old plant). Measure the length of each individual with a cm ruler. Select the flower bud of 17-22 mm in length when the length of the sepals equals that of [...]