A compound microscope is an indispensable instrument in any biological laboratory. It is used for passive observation of structural details of a cell, tissue or organ in sections. A modern compound microscope has following structural components. Non-Optical Components: 1. Base (foot): It is U or horseshoe-shaped metallic structure that supports the whole microscope. 2. Pillar: It is a short upright [...]
Fundamentals of Light Microscopy and Transmission Electron Microscopy: Until the 1940s, most of our knowledge concerning the structure and organization of cells was obtained from studies conducted using light microscopy, and major structures and organelles, including the cell wall, nuclei, chromosomes, chloroplasts, mitochondria, vacuoles, centrioles, flagella, and cilia, had been described.The smallest distance, d, between two points resolvable as separate [...]
Phase Contrast Microscope: Short notes on Phase Contrast Microscope ! In recent years, remarkable advances have been made in the study of living cells (unstained) by the development of special optical techniques such as phase contrast and interference microscopy. The biological specimens are highly transparent to visible light and they cause phase changes in transmitted radiations. The phase contrast microscope [...]
