In this article we will discuss about the analysis of promoter methylation using Denaturing High-Performance Liquid Chromatography (DHPLC). Introduction to Analysis of Promoter Methylation using DHPLC: Sequencing and analysis of the human genome revealed the presence of only 30,000 to 40,000 protein-coding genes, a stark contrast to previous estimates of about 100,000 genes. While a large number of putative tumor [...]
In this article we will discuss about the rapid analysis to detect base changes in human mitochondrial DNA by restriction enzyme analysis followed by Denaturing High Performance Liquid Chromatography (DHPLC). Introduction to Mitochondrial Genome Analysis: Mitochondria are DNA-containing organelles found within the cytoplasm of eukaryotic cells. Their main function is to provide energy for cellular activity in the form of [...]
In this article we will discuss about the mutation analysis of the BRCA1 and BRCA2 genes. The BRCA Genes and Hereditary Breast and Ovarian Cancer: During the last decade several genes responsible for autosomal dominant transmission of greatly increased risk of specific cancers were identified. Although it has been known for years that some families exhibited an apparÂently autosomal dominant [...]