In this article we will discuss about the influence of PCR on DHPLC polymorphism characterization. Introduction: Denaturing high performance liquid chromatography (DHPLC) has emerged as a powerful tool to detect polymorphisms and mutations in genomes of living organisms. Relative to other mutation detection techniques such as sequencing, primer extension, and SSCP, DHPLC is: 1. Sensitive: Clear separation between signal and [...]
In this article we will discuss about the mutation detection in the Tuberous Sclerosis Genes TSC1 and TSC2 by DHPLC. Overview of Tuberous Sclerosis (TSC): TSC is a relatively variable clinical syndrome, in which several pathologic manifestations are highly specific for the disease. The cardinal feature is the cortical tuber, which is a distinctive form of brain hamartia. Hamartias and [...]
In this article we will discuss about SIRPH - A Rapid, Quantitative SNuPE-IP RP HPLC Method for Simultaneous Methylation Analysis at Multiple CpG Sites. Introduction: Bisulfite Based DNA Methylation Analysis: The most widely used protocols for detailed DNA methylation analyses are based on the use of bisulfite converted genomic DNA. This methodology was introduced by M. Frommer in the early [...]