In this article we will discuss about the mutation detection in the Tuberous Sclerosis Genes TSC1 and TSC2 by DHPLC. Overview of Tuberous Sclerosis (TSC): TSC is a relatively variable clinical syndrome, in which several pathologic manifestations are highly specific for the disease. The cardinal feature is the cortical tuber, which is a distinctive form of brain hamartia. Hamartias and [...]
In this article we will discuss about the novel DHPLC based strategy for identification and characterisation of (BI)-Clonal T-Cell receptor targets for the detection of minimal residual disease in childhood acute lymphoblastic leukemia. Introduction: Clonal antigen receptor rearrangements are widely used as patient-specific markers for detection of minimal residual disease (MRD) in acute lymphoblastic Leukemia (ALL). Since ALL blasts are [...]
In this article we will discuss about the analysis of promoter methylation using Denaturing High-Performance Liquid Chromatography (DHPLC). Introduction to Analysis of Promoter Methylation using DHPLC: Sequencing and analysis of the human genome revealed the presence of only 30,000 to 40,000 protein-coding genes, a stark contrast to previous estimates of about 100,000 genes. While a large number of putative tumor [...]