Archive | Genetics

Novel and Innovative Applications of DHPLC | Genetics | Biology

In this article we will discuss about the novel DHPLC based strategy for identification and characterisation of (BI)-Clonal T-Cell receptor targets for the detection of minimal residual disease in childhood acute lymphoblastic leukemia. Introduction: Clonal antigen receptor rearrangements are widely used as patient-specific markers for detection of minimal residual disease (MRD) in acute lymphoblastic Leukemia (ALL). Since ALL blasts are [...]

By |2017-03-15T04:10:06+00:00March 15, 2017|Genetics|Comments Off on Novel and Innovative Applications of DHPLC | Genetics | Biology
Read More

Influence of PCR on DHPLC | Genetics | Biology

In this article we will discuss about the influence of PCR on DHPLC polymorphism characterization. Introduction: Denaturing high performance liquid chromatography (DHPLC) has emerged as a powerful tool to detect polymorphisms and mutations in genomes of living organisms. Relative to other mutation detection techniques such as sequencing, primer extension, and SSCP, DHPLC is: 1. Sensitive: Clear separation between signal and [...]

By |2017-03-15T04:10:06+00:00March 15, 2017|Genetics|Comments Off on Influence of PCR on DHPLC | Genetics | Biology
Read More

DNA Methylation Analysis by DHPLC | Genetics | Biology

In this article we will discuss about SIRPH - A Rapid, Quantitative SNuPE-IP RP HPLC Method for Simultaneous Methylation Analysis at Multiple CpG Sites. Introduction: Bisulfite Based DNA Methylation Analysis: The most widely used protocols for detailed DNA methylation analyses are based on the use of bisulfite converted genomic DNA. This methodology was introduced by M. Frommer in the early [...]

By |2017-03-15T04:10:05+00:00March 15, 2017|Genetics|Comments Off on DNA Methylation Analysis by DHPLC | Genetics | Biology
Read More
Go to Top