The mechanism of protein synthesis has been studied thoroughly in E.coli. The process of protein synthesis on 70S ribosomes is described in detail below. The summary of the various steps in the mechanism of protein synthesis is shown in (Fig. 13). Step 1 — Transcription: One strand of DNA molecule functions as a template for the formation of mRNA. This [...]
In this article we will discuss about the basics of mutation screening by Denaturing High-Performance Liquid Chromatography (DHPLC). Introduction to Basics of Mutation by DHPLC: Studies of sequence variation are an integral part of genome research, because of differences in the genomic sequences between individuals. Variations in mammalian genomic sequences occur at an estimated frequency of at least one base [...]
In this article we will discuss about the novel DHPLC based strategy for identification and characterisation of (BI)-Clonal T-Cell receptor targets for the detection of minimal residual disease in childhood acute lymphoblastic leukemia. Introduction: Clonal antigen receptor rearrangements are widely used as patient-specific markers for detection of minimal residual disease (MRD) in acute lymphoblastic Leukemia (ALL). Since ALL blasts are [...]