The mechanism of protein synthesis has been studied thoroughly in E.coli. The process of protein synthesis on 70S ribosomes is described in detail below. The summary of the various steps in the mechanism of protein synthesis is shown in (Fig. 13). Step 1 — Transcription: One strand of DNA molecule functions as a template for the formation of mRNA. This [...]
In this article we will discuss about the basics of mutation screening by Denaturing High-Performance Liquid Chromatography (DHPLC). Introduction to Basics of Mutation by DHPLC: Studies of sequence variation are an integral part of genome research, because of differences in the genomic sequences between individuals. Variations in mammalian genomic sequences occur at an estimated frequency of at least one base [...]
In this article we will discuss about the influence of PCR on DHPLC polymorphism characterization. Introduction: Denaturing high performance liquid chromatography (DHPLC) has emerged as a powerful tool to detect polymorphisms and mutations in genomes of living organisms. Relative to other mutation detection techniques such as sequencing, primer extension, and SSCP, DHPLC is: 1. Sensitive: Clear separation between signal and [...]