In this article we will discuss about the production of various enzymes. 1. Pectinases: During the survey of crops for disease occurrence, it was observed that certain soft-rot causing bacteria produced pectolytic enzymes.The wilt disease induced by Fusarium species also produced pectinases as observed by Gothoskar 1955 and Waggoner and Dimond (1955). It was Scheffer and others who in the [...]
In this article we will discuss about:- 1. Meaning of Restriction Enzymes 2. Naming of Restriction Enzymes 3. Mechanism of Action. Meaning of Restriction Enzymes: These are also known as molecular scissors, used for cutting of DNA. The cutting of DNA at specific locations became possible with the discovery of molecular scissors, i.e., restriction enzymes. In the year 1963, the [...]
In this article you will discuss about the tests for estimation of catalase in plants. Principle: The enzyme catalase is responsible for catalysing the decomposition of H2O into H2O and O2. The assay of enzyme depends on estimation of residual H2O2 by titration with KMnO4. Requirements: (a) Reagents: 1. H2O2 —0.2 volume 2. H2SO4—10% 3. 0.05 NKMnO4 solution. (b) Glass-wares: [...]