An exclusive project report on Recombinant DNA Technology. This project report will help you to learn about: 1. Introduction to Recombinant DNA Technology 2. Purpose of Recombinant DNA Technology 3. Basic Steps 4. Enzymes Involved 5. Vectors Used. Contents: Project Report on Introduction to Recombinant DNA Technology Project Report on the Purpose of Recombinant DNA Technology Project Report on the [...]
In this article we will discuss about the Insertion of a Foreign DNA Fragment into a Vector. Introduction to the Insertion of a Foreign DNA Fragment into a Vector: The specific section of DNA generated by the action of restriction enzyme is spliced or incorporated into an agent called cloning vehicle. Cloning vehicle is also called vector. Cloning vehicles or [...]
The following points highlight the top three methods of recombinant DNA formation. The methods are: 1. Transformation 2. Transfection 3. Non-Bacterial Transformation. Recombinant DNA Formation: Method # 1. Transformation: The restriction enzyme which causes a break in foreign DNA also causes a staggered cut in the vector DNA at a specific cleavage site. The cohesive ends of vector DNA possess [...]