In this article we will discuss about the Polymerase Chain Reaction:- 1. Steps of Polymerase Chain Reaction 2. Advantages of Polymerase Chain Reaction 3. Applications. Steps of Polymerase Chain Reaction: PCR uses DNA polymerase to amplify repetitively targeted portions of DNA. The amplified DNA sequence can then be analysed by southern hybridization. i. Primer Construction: (a) It is essential to [...]
The below mentioned article provides a study note on the Prenatal Diagnosis of Genetic Diseases. It is required to analyse DNA of family members of the affected individual to set up a diagnostic tool for a genetic disease. The markers (RFLPs) that are tightly linked to the disease is needed to be identified first. The physicians are required to have [...]
The following points highlight the three main molecular basis of inherited DNA diseases. The molecular basis are: 1. Genetic Diseases 2. Cleavage of DNA into Fragments 3. DNA Cloning. Molecular Basis # 1. Genetic Diseases: The human genome contains DNA with about 3 billion (109) base pairs that encode 50,000 to 100,000 genes located on 23 pairs of chromosomes. The [...]