Read this article to learn about the principle of recombinant DNA technology. The principle of recombinant DNA technology involved four steps. The four steps are: (1) Gene Cloning and Development of Recombinant DNA (2) Transfer of Vector into the Host (3) Selection of Transformed Cells and (4) Transcription and Translation of Inserted Gene. Knowledge about cell and its functioning has [...]
This article throws light upon the three types of cloning vectors used in recombinant DNA technology. The three types of cloning vectors are: (1) Bacteriophage (2) Phagemids and (3) Cosmids. Type # 1. Bacteriophage as Cloning Vectors: The plasmid based vectors used for cloning DNA molecules generally carry up to 10 kb of inserted DNA. However, for the formation of [...]
Read this article to learn about the restriction enzymes and their mode of action. And it also describes different types of restriction enzymes. The types are: (1) Type I (2) Type II and (3) Type III. Restriction enzymes are DNA-cutting enzymes found in bacteria (and harvested from them for use). Because they cut within the molecule, they are often called [...]