In this article we will discuss about the procedure for cloning recombinant DNA. The first step in a gene cloning programme is to construct a recombinant DNA molecule containing a donor DNA segment in which a selected gene is located and a vector DNA. The donor may belong to any taxonomic group. The DNA of the donor is cleaved into [...]
In this article we will discuss about the properties and functions of genetic material in organisms. The genetic material must have some well-defined properties to fulfill its functions. These are enumerated below: 1. It must act as a store-house of information and must be able to transmit the information to the biochemical machinery of the cell. 2. It must be [...]
In this article we will discuss about the three main types of vectors used in cloning for larger molecules of DNA. The types of vectors are: 1. Plasmid Vectors 2. Bacteriophage Vectors 3. Cosmid Vectors. 1. Plasmid Vectors: Plasmids are the extra chromosomal genetic elements commonly found in bacteria and are mostly made of double-stranded circular DNA. They are used [...]