In this article we will discuss about the three main types of vectors used in cloning for larger molecules of DNA. The types of vectors are: 1. Plasmid Vectors 2. Bacteriophage Vectors 3. Cosmid Vectors. 1. Plasmid Vectors: Plasmids are the extra chromosomal genetic elements commonly found in bacteria and are mostly made of double-stranded circular DNA. They are used [...]
In this article we will discuss about the top two techniques used for introduction of foreign DNA without vectors. The techniques are: 1. Electroporation Technique 2. Microinjection Technique Some direct methods have also been developed for transferring DNA of one organism to a recipient without the participation of a vector. Two such methods are: 1. Electroporation technique and 2. Microinjection [...]
In this article we will discuss about the procedure for cloning recombinant DNA. The first step in a gene cloning programme is to construct a recombinant DNA molecule containing a donor DNA segment in which a selected gene is located and a vector DNA. The donor may belong to any taxonomic group. The DNA of the donor is cleaved into [...]