In this article we will discuss about a generalised model of DNA replication in prokaryotes. DNA replication is catalyzed by the enzyme DNA polymerase, and it involves several other proteins/enzyme. The data collected from both in vitro and in vivo studies have been synthesized into a generalized model DNA replication, a brief and simplified description of which is given below [...]
Theoretically, there are the following three possible modes of DNA replication: (1) Dispersive, (2) Conservative and (3) Semi-conservative. 1. Dispersive Replication: In dispersive replication, the parent DNA molecule is broken into several pieces, and the 'new' molecules will consist of both old and newly synthesized segments. In the conservative scheme, the two 'old' strands would be present in one daughter [...]
In this article we will discuss about the top five techniques used for cloning of specific genes and their identification. The techniques are: 1. Direct Selection of Recombinant Clones 2. Colony Hybridization 3. Complementary DNA (c-DNA) 4. Isolation of a Specific Gene: Southern Blotting 5. Polymerase Chain Reaction (PCR). The restriction digest always contains an assortment of donor DNA fragments [...]