The following points highlight the four main features that are required to facilitate cloning into a vector. The features are: 1. Origin of Replication 2. Selectable Marker 3. Cloning (Recognition) Sites 4. Vectors for Cloning Genes in Plants and Animals. Feature # 1. Origin of Replication (Ori): This is a sequence from where replication starts and any piece of DNA, [...]
The below mentioned article provides a note on DNA:- 1. DNA Chemistry 2. DNA Replication 3. Carrier of Genetic Information. DNA Chemistry: DNA is found to consist of thread-like particles of high molecular weight in isolated condition. A number of units called nucleotides become linked together and form the nucleic acid. A nucleotide is composed of a molecule of Pentose [...]
In this article we will discuss about the steps and applications of Recombinant DNA Technology. Steps Involved in Recombinant DNA Technology: The sequences of steps in r-DNA technology are as follows: (i) Cell culture of cells with the required DNA sequences is obtained through tissue culture technique, (ii) Specific restriction endonuclease is selected from the specific bacterium, (iii) Restriction endonuclease [...]