In this article we will discuss about the gene rearrangements in light and heavy chain DNAs. Rearranged k and λ genes of light chain contain gene segments in the order from 5' to 3' end- a short leader (L) gene segment, an intron (noncoding sequence), a joined VJ-gene segment, a second intron, and a C-gene segment (Fig. 22.18). The rearranged [...]
The below mentioned article provides a diagram of DNA strand. (a) DNA is made up of two polynucleotide chains. The backbone is constituted by sugar-phosphate, while the nitrogenous bases project inwards. (b) The two chains have anti-parallel polarity. Therefore, when one chain has 3' → 5'polarity, the other chain has 5' → 3' polarity. (c) The bases in two strands [...]
The following points highlight the seven main stages of recombinant DNA technology. The stages are: 1. Isolation of the Genetic Material (DNA) 2. Cutting of DNA at Specific Locations 3. Isolation of Desired DNA Fragment 4. Amplification of Gene of Interest using PCR 5. Ligation of DNA Fragment into a Vector 6. Insertion of Recombinant DNA into the Host Cell/Organisms [...]