The process of DNA cloning is carried out in four ways: 1. DNA Cloning Inside Host Cell 2. Chemically by Polymerase Chain Reaction (PCR) 3. Reproductive Cloning 4. Therapeutic Cloning. Way # 1. DNA Cloning Inside Host Cell: DNA cloning is the selective amplification of a specific DNA fragment or a sequence for producing large quantities of the DNA fragment [...]
Introduction to Recombinant DNA Technology: Recombinant DNA technology has revolutionised life sciences, opening new vistas for research in molecular biology. It allows genetic manipulation using techniques for synthesizing, amplifying and purifying individual genes from any type of cell. Genomics has emerged as an extension of recombinant DNA technology for high resolution mapping and characterization of whole genomes and gene products [...]
In this article we will discuss about the applications of PCR. i. Detection of mutations: PCR is useful for detection of mutations related to genetic disease including point mutations, insertions and deletions. A notable example is that of the human dystrophin gene associated with Duchenne muscular dystrophy. The large-sized dystrophin gene consists of about two million base pairs located on [...]