The Watson-Crick model of DNA has been the basis for further work on DNA replication, mutation and recombination. Much evidence exists to establish the validity of this model. Nevertheless, the model has been scrutinized by other workers, and from time to time other models have been suggested by Wu (1970), Arnott (1968), Wang (1979) and a few others. Wang's model [...]
In this article we will discuss about the repeated sequence of chromosomal DNA. Eukaryotic genomes contain large amount of repetitive sequences, sometimes present in hundreds or thousands of copies per genome. The understanding of repetitive sequences is based on studies conducted on denaturation (separation of DNA double helix into its two component strands) and renaturation (re-association of the single strands [...]
In this article we will discuss about the applications of PCR. i. Detection of mutations: PCR is useful for detection of mutations related to genetic disease including point mutations, insertions and deletions. A notable example is that of the human dystrophin gene associated with Duchenne muscular dystrophy. The large-sized dystrophin gene consists of about two million base pairs located on [...]