In this article we will discuss about the DNA replication in eukaryotes. In eukaryotes there are only two different types of DNA polymerases in contrast with DNA polymerase I, II and III of prokaryotes. Furthermore the DNA of eukaryotes is a long linear molecule with several replication units. A diploid mammalian cell contains on an average about 6 pg of [...]
In this article we will discuss about the evidence that DNA is the genetic material in viruses. Is DNA The conclusion reached by Avery, Macleod and McCarty was not immediately accepted. Some investigators suggested that the DNA preparations contained a mutagen which caused mutation to the S form. Others pointed out that transformation was due to traces of some specific [...]
The work of J. Cairns demonstrated DNA replication in bacteria in an elegant way. He used high resolution autoradiography for observing the distribution of radioactive label in the "chromosomes" of dividing E. coli cells. The bacterial cells were grown on a medium containing tritiated thymidine. The labelled DNA was extracted from the cells and spread on EM grids. A very [...]