In this article we will discuss about the introduction and mechanism of DNA replication. Introduction to DNA Replication: While presenting the double helical model of DNA, Watson and Crick had remarked, "It has not escaped our notice that the specific pairing (of bases) we have postulated suggests a possible copying mechanism for the genetic material." The complementary nature of the [...]
In this article we will discuss about the evidence that DNA is the genetic material in viruses. Is DNA The conclusion reached by Avery, Macleod and McCarty was not immediately accepted. Some investigators suggested that the DNA preparations contained a mutagen which caused mutation to the S form. Others pointed out that transformation was due to traces of some specific [...]
The work of J. Cairns demonstrated DNA replication in bacteria in an elegant way. He used high resolution autoradiography for observing the distribution of radioactive label in the "chromosomes" of dividing E. coli cells. The bacterial cells were grown on a medium containing tritiated thymidine. The labelled DNA was extracted from the cells and spread on EM grids. A very [...]