In this article we will discuss about the uses of DNA microarrays. The DNA Microarray technology is used to determine the level of expression of many thousands of genes simultaneously. This new approach is used not for individual genetic loci, rather, for the analysis of genome-wide patterns of gene expression. Using DNA microarrays, it is possible to estimate the relative [...]
The process of DNA cloning is carried out in four ways: 1. DNA Cloning Inside Host Cell 2. Chemically by Polymerase Chain Reaction (PCR) 3. Reproductive Cloning 4. Therapeutic Cloning. Way # 1. DNA Cloning Inside Host Cell: DNA cloning is the selective amplification of a specific DNA fragment or a sequence for producing large quantities of the DNA fragment [...]
Recombinant DNA has opened new avenues for the study of gene function. The function of a cloned gene can be investigated by introducing the cloned DNA into eukaryotic cells. In simple eukaryotes such as yeasts Saccharomyces cerevisiae, molecular clones of various mutants have been prepared for genetic analysis. Like bacteria, yeasts offer advantages in having a small genome of about [...]