Volumetric oxygen transfer coefficient (KLa) serves as a determinant of aeration efficiency of a bioreactor. Maintenance of uniform KLa throughout the liquid is most important in aerobic submerged fermentation. In fact KLa is influenced by several factors as described in the following sections: 1. Broth viscosity: A relation between apparent viscosity (ηapp) and KLa for penicillin fermentation broth was provided [...]
Read this article to learn about 3 Methods of Measuring Dissolved Oxygen! 1. Chemical Method: The determination of DO concentration in pure water or in waste water involves the addition of known excess quantities of a standard solution of reduced ion (such as ferrous or manganous), followed by a back titration of the excess with a known standard oxidizing agent. [...]
In oxic bioprocess engineering system in liquid culture transfer of oxygen from air to liquid has been measured by fast response steam sterilizable dissolved oxygen probe on line. In the measurement, the concept of flow of oxygen from higher concentration (heaviside) to lower concentration has been conceived. 1. Stagnant Film Concept: Whitman based his film theory on Fick's first law. [...]