Archive | Cell Biology

The presence of reactive oxygen species can be monitored both directly and indirectly. Several direct methods are available which include electron spin resonance spectroscopy, chemiluminescence detection, pulse radiolysis, and high-performance liquid chromatography. Oxygen free radicals once formed in a biological system attack the membrane lipids causing the peroxidation. The products of peroxidation can also be estimated as an indirect method [...]

In general, a conventional submerged type of bioreactor/oxygenator with agitation system has been used in cell cultivations. The same type of oxygenator as used for the homogeneous cultivation of microorganisms may be applied for plant cell cultivation and for micro-carrier supported animal cell culture. There are three major types of homogeneous-oxygenator systems used for propagating microbial, plant, and animal cells. [...]

1. Chemical Method: The chemical method is known as the sulfite oxidation method. It involves the determination of the maximum rate of oxidation of sodium sulfite to sodium sulfate in the presence of c0so4 or CuSO4 catalyst, in which there is no back pressure of dissolved oxygen. The reaction is independent of sulfite concentration in the range of 0.8 M [...]