1. DNA-DNA hybridization/rDNA technology: In general when a double stranded DNA is heated, the two strands separate into single stranded molecules. On cooling re-association of the two complementary strands slowly takes place, these results in the formation of the original double stranded DNA complex. If single stranded DNAs from two different microorganisms are mixed and the nucleotide sequences are identical [...]
1. General: Growth related intracellular events as shown in Fig. 2.4 are the following: (a) Anabolic aberration and spontaneous mutations. (b) Biosynthetic error. (c) Post biosynthetic damage in cells. During growth of cells intracellular proteins in wild microbial strains are broken down to appropriate rates during starvation or endogenous metabolism. Even in exponential phase of growth of bio cells in [...]
1. Cooper-Helmstetter concept: When a bacteria divides more frequently than at 60 minutes intervals, a cycle of replication is initiated before the end of every 35 minutes. This may be taken as an example. The cycle of replication connected with a division must have been initiated 25 minutes before the preceding division. This situation as presented in literature shows the [...]