Quick Notes on Isolation of Protoplasts and Protoplast Culture
The below mentioned article provides a quick notes on Isolation of Protoplasts and Protoplast Culture. Klerker (1892) made unsuccessful attempts to isolate protoplasts. In 1909 Kunster isolated protoplasts by mechanical method. Effective technique of protoplast isolation was evolved by Cocking ('60), Nagata and Takebe ('70) and Bhojwani and Cocking ('72). Enzymes used for isolation of protoplasts are cellulase, hemicellulase and [...]