Quick Notes on Isolation of Protoplasts and Protoplast Culture

The below mentioned article provides a quick notes on Isolation of Protoplasts and Protoplast Culture. Klerker (1892) made unsuccessful attempts to isolate protoplasts. In 1909 Kunster isolated protoplasts by mechanical method. Effective technique of protoplast isola­tion was evolved by Cocking ('60), Nagata and Takebe ('70) and Bhojwani and Cocking ('72). Enzymes used for isolation of protoplasts are cellulase, hemicellulase and [...]