The following points highlight the nine main techniques of protoplast culture. The techniques are: 1. Agar Plating Method 2. Micro-Chamber 3. Nurse Culture 4. Immobilization of Protoplast 5. Nutrient Requirement 6. Growth Regulators 7. Cultural Conditions 8. Cell Wall Regeneration 9. Regeneration. Technique # 1. Agar Plating Method: Protoplast can be cultured conveniently by using agar plating method. Two millilitres [...]
In this article we will discuss about the significance of protoplast culture. Cell Modification by DNA Uptake: The isolated plant protoplasts are best suited for the introduction of foreign genetic material. Protoplast acts as an efficient receptor for the uptake of foreign DNA. Protoplast can be regenerated into the whole plant and this is indispensable for genetic manipulation. The ratio [...]
In this article we will discuss about:- 1. Sources of Explant for Protoplast Isolation 2. Methods of Protoplast Isolation 3. Purification of Protoplasts 4. Isolation of Sub-Protoplasts 5. Protoplast Viability Test. Each plant cell has a definite cellulose wall which is attached with each other by middle lamella made up of pectin. Protoplast is the content which is surrounded only [...]