The following points highlight the nine main techniques of protoplast culture. The techniques are: 1. Agar Plating Method 2. Micro-Chamber 3. Nurse Culture 4. Immobilization of Protoplast 5. Nutrient Requirement 6. Growth Regulators 7. Cultural Conditions 8. Cell Wall Regeneration 9. Regeneration. Technique # 1. Agar Plating Method: Protoplast can be cultured conveniently by using agar plating method. Two millilitres [...]
In this article we will discuss about the significance of protoplast culture. Cell Modification by DNA Uptake: The isolated plant protoplasts are best suited for the introduction of foreign genetic material. Protoplast acts as an efficient receptor for the uptake of foreign DNA. Protoplast can be regenerated into the whole plant and this is indispensable for genetic manipulation. The ratio [...]
In this article we will discuss about the stages and methods of protoplast culture. Isolated protoplasts are cultured either in a liquid medium or semisolid agar medium in a thin layer or as small drops of nutrient medium in petridish. The medium for protoplast culture requires the same component as required for callus or suspension culture. Increasing the calcium concentration [...]