Tag Archives | Protoplast Culture

Protoplast Culture: Stages and Methods | Biotechnology

In this article we will discuss about the stages and methods of protoplast culture. Isolated protoplasts are cultured either in a liquid medium or semisolid agar medium in a thin layer or as small drops of nutrient medium in petridish. The medium for protoplast culture requires the same component as required for cal­lus or suspension culture. Increasing the calcium concentration [...]

By |2016-11-02T05:49:29+00:00November 2, 2016|Protoplasts|Comments Off on Protoplast Culture: Stages and Methods | Biotechnology
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Top 8 Practical Applications of Protoplast Culture | Biotechnology

The following points highlight the top eight practical applications of protoplast culture. Application # 1. Production of Somatic Hybrids: To overcome the sexual incompatibility barriers the protoplast fusion programme may be taken to obtain the interspecific and inter-generic hybrids. For example, the fusion between Lycopersicon esculentum (tomato) and Solanum tuberosum (potato) have been possible to obtain the somatic hybrid 'Pomato', [...]

By |2016-11-02T05:49:28+00:00November 2, 2016|Protoplasts|Comments Off on Top 8 Practical Applications of Protoplast Culture | Biotechnology
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