In this article we will discuss about the stages and methods of protoplast culture. Isolated protoplasts are cultured either in a liquid medium or semisolid agar medium in a thin layer or as small drops of nutrient medium in petridish. The medium for protoplast culture requires the same component as required for calÂlus or suspension culture. Increasing the calcium concentration [...]
The following points highlight the top eight practical applications of protoplast culture. Application # 1. Production of Somatic Hybrids: To overcome the sexual incompatibility barriers the protoplast fusion programme may be taken to obtain the interspecific and inter-generic hybrids. For example, the fusion between Lycopersicon esculentum (tomato) and Solanum tuberosum (potato) have been possible to obtain the somatic hybrid 'Pomato', [...]
In this article we will discuss about:- 1. Method of Protoplast Fusion 2. Mechanism of Protoplast Fusion 3. Identification and Selection 4. Verification and Characterisation. Isolated protoplasts are devoid of cell walls which make them easy tools for underÂgoing fusions in vitro. An important factor is that generally there is incompatibility barrier between two protoplasts of different species or genera. [...]