The following points highlight the ten important procedures of hybrid selection. The procedures are: 1. Auxin Autotrophy 2. Use of Genetic Complementation 3. Use of Uncommon Amino Acids 4. Use of Cells Resistance to Amino Analog 5. Use of Phytotoxin 6. Use of Antibiotics 7. Use of Auxotrophic Mutant 8. Use of Metabolic Mutant 9. Using Isoenzyme Analysis 10. Use [...]
This article throws light upon the three important applications of protoplasts hybridization. The three important applications are: (A) Hybrids and Cybrids Production (B) Asymmetric Hybrids Production and (C) Direct DNA and Macromolecules Uptake. A. Hybrids and Cybrids Production: Protoplasts fusion technique during 1970s established that Para sexual hybrids can be produced by this method. Intra and inter-specific hybrids have been [...]
Read this article to learn about the importance, isolation techniques, culture and regeneration of protoplasts and also learn about sub-protoplasts. Sub-protoplasts is divided into three types. The three types are: (1) Mini-protoplasts (2) Cytoplasts and (3) Micro-protoplasts. Protoplasts are naked plant cells without the cell wall, but they possess plasma membrane and all other cellular components. They represent the functional [...]