In this article we will discuss about the colorimetric method for estimation of amino acids in plants. Principle: Amino acid content can be estimated quantitatively by Colorimetric method using ninhydrin reagent. The reaction of ninhydrin (triketohydrindene hydrate) with amino acids is of particular importance in the regard. Ninhydrin is a powerful oxidizing agent and elicits the oxidative deamination of the [...]
In this article we will discuss about the tests for estimation of ascorbic acid in plants. Principle: Ascorbic acid oxidase acts on the substrate ascorbic acid to form dehydro-ascorbic acid. The residual ascorbic acid content is determined by titration using a dye solution (Dichlorophenol indophenol, an oxidation-reduction dye): Requirements: (a) Reagents: 1. Dye solution: 15 mgm of 2-6 dichlorophenol indophenol [...]
In this article we will discuss about the tests for detection of tannins in plants. Tannins are a heterogeneous group of organic compounds found in plants. They are commonly present in unripe fruits — guava, banana and leaves of tea etc. These are water-soluble compounds. The metabolic significance of these compounds are not clearly known but they play a significant [...]