Tag Archives | Practicals

Estimation of Ascorbic Acid | Enzyme Activity

In this article we will discuss about the tests for estimation of ascorbic acid in plants. Principle: Ascorbic acid oxidase acts on the substrate ascorbic acid to form dehydro-ascorbic acid. The residual ascorbic acid content is determined by titration using a dye solution (Dichlorophenol indophenol, an oxidation-reduction dye): Requirements: (a) Reagents: 1. Dye solution: 15 mgm of 2-6 dichlorophenol indophenol [...]

By |2016-10-20T07:40:08+00:00October 20, 2016|Plants|Comments Off on Estimation of Ascorbic Acid | Enzyme Activity

Detection of Fats and Oils in Plants

In this article we will discuss about the tests for detection of fats and oils in plants with the help of experiments. Fats and Oils are invariably present in all living cells. They are synthesized through an active metabolism and also serve a number of indispensable roles in physiological processes. Fats are the esters of fatty acids with glycerol and [...]

By |2016-10-20T07:40:07+00:00October 20, 2016|Plants|Comments Off on Detection of Fats and Oils in Plants

Estimation of Amylase | Enzyme Activity

In this article we will discuss about the tests for estimation of amylase in plants. Principle: The reducing sugars produced by the action of α- and or β-amylase react with di-nitro-salicylic acid and reduce it to a brown coloured product, nitro-amino-salicylic acid. Materials: i. Sodium acetate buffer, 0.1 M pH 4.7 ii. Starch 1% Solution: Prepare a fresh solution by [...]

By |2016-10-20T07:40:07+00:00October 20, 2016|Plants|Comments Off on Estimation of Amylase | Enzyme Activity
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