In this article we will discuss about the tests for detection of tannins in plants. Tannins are a heterogeneous group of organic compounds found in plants. They are commonly present in unripe fruits — guava, banana and leaves of tea etc. These are water-soluble compounds. The metabolic significance of these compounds are not clearly known but they play a significant [...]
In this article we will discuss about the tests for estimation of amylase in plants. Principle: The reducing sugars produced by the action of α- and or β-amylase react with di-nitro-salicylic acid and reduce it to a brown coloured product, nitro-amino-salicylic acid. Materials: i. Sodium acetate buffer, 0.1 M pH 4.7 ii. Starch 1% Solution: Prepare a fresh solution by [...]
A number of inorganic elements are present in plants which also catalyse many enzymatic reactions. The existence of such inorganic elements in plants can be detected by the chemical tests of plant ash. Requirements for Detection of Ash: (a) Preparation of Ash Extract: For detection of various elements in plant ash viz., Ca, Mg, Fe, S, P, CI etc. ash [...]