In this article we will discuss about the complexometric method for estimation of calcium and magnesium in plants. Principle: Many complex-forming ions or molecules lead to a stepwise formation of complexes. Each complex is characterized by its own stability constant. This property of stepwise formation of complex can be used in titrimetry and, thus, one can be able to estimate [...]
In this article we will discuss about the tests for detection of fats and oils in plants with the help of experiments. Fats and Oils are invariably present in all living cells. They are synthesized through an active metabolism and also serve a number of indispensable roles in physiological processes. Fats are the esters of fatty acids with glycerol and [...]
In this article we will discuss about the tests for estimation of amylase in plants. Principle: The reducing sugars produced by the action of α- and or β-amylase react with di-nitro-salicylic acid and reduce it to a brown coloured product, nitro-amino-salicylic acid. Materials: i. Sodium acetate buffer, 0.1 M pH 4.7 ii. Starch 1% Solution: Prepare a fresh solution by [...]