In this article we will discuss about the tests for estimation of lipase in plants. Principle: The quantity of fatty acid released in unit time is measured by the quantity of NAOH required to maintain pH constant. The milliequivalent of alkali consumed is taken as a measure of the activity of the enzyme. Materials: a. Substrate: Take 2 ml of [...]
In this article we will discuss about the tests for estimation of cellulase in plants. Principle: Endo-β-1, 4 glucanase acts on carboxymethyl cellulose (CMC) and hydrolyses the β-1, 4 glycosidic bonds in a random manner. As a result, the viscosity of CMC solution is reduced. This is measured in an Ostwald viscometer. Materials: a. Citrate-phosphate Buffer 0.1 M (pH 6.0) [...]
In this article we will discuss about the Kjeldahl method for the estimation of nitrogen in plants. Principle: Digestion of nitrogenous compounds by concentrated sulphuric acid in presence of catalysts (reduced iron powder, and a mixture of CuSO4 and K2SO4) produces ammonium compound. Further, such ammonium compound when distilled by steam in presence of strong alkali (40% NaOH soln.) produces [...]