In this article we will discuss about the tests for estimation of free fatty acid. Principle: The free fatty acid in an oil is estimated by titrating it against KOH in the presence of phenolphthalein indicator. The acid number is defined as the mg KOH required to neutralize the free fatty acids present in 1 g of sample. However, the [...]
In this article we will discuss about the tests for estimation of lipase in plants. Principle: The quantity of fatty acid released in unit time is measured by the quantity of NAOH required to maintain pH constant. The milliequivalent of alkali consumed is taken as a measure of the activity of the enzyme. Materials: a. Substrate: Take 2 ml of [...]
In this article we will discuss about the colorimetric method for estimation of phosphorus in plants. Principle: When ammonium molybdate solution is added to a solution of phosphate containing conc. H2SO4 it produces a yellow crystalline precipitation of ammonium phospho-molybdate. Phospho-molybdate reacts with amino-naphthol-sulphonic acid and produces a molybdenum complex which forms a blue- coloured solution. The appearance of colour [...]