The following points highlight the top four methods of chromosome studies. The methods are: 1. Fixation 2. Stains 3. Pre-Treatment 4. Method of Making Slides Grease-Free. Chromosome Studies: Method # 1. Fixation: Fixation may be defined as the selective preservation of morphological organization and chemical composition of tissues for microscopic observation. The main effects of fixative are — rendering the [...]
The below mentioned article provides a practical guide to microscopes, drawing appliances and measurement. Light Microscope: The different components of a light microscope are shown in Fig. 1.1. The path of light through a microscope is shown in Fig. 1.2. The most important parts of a microscope are its lenses, of which there are two — objective and eye-piece (ocular), [...]
The below mentioned article provides study notes on Microtome Sections:- 1. Section-Cutting 2. Mounting of Sections 3. Stretching of Sections 4. Drying of Sections 5. Staining the Sections 6. Staining the Sections 7. Schedule of Staining the Section. Microtome Sections # Section-Cutting: Rotary microtomes are commonly used for section-cutting. Move the block holding the socket backward as far as possible [...]