The below mentioned article provides study notes on Microtome Sections:- 1. Section-Cutting 2. Mounting of Sections 3. Stretching of Sections 4. Drying of Sections 5. Staining the Sections 6. Staining the Sections 7. Schedule of Staining the Section. Microtome Sections # Section-Cutting: Rotary microtomes are commonly used for section-cutting. Move the block holding the socket backward as far as possible [...]
In this article we will discuss about the preparation of paraffin block. Transfer the tube or tubes containing the material to a high temperature paraffin bath (60°C), along with some paraffin of suitable melting point taken in a beaker. Suitable melting point refers to the melting point of paraffin which will give ideal ribbons of sections (see section cutting). This [...]
In this article we will discuss about the chi-square method for testing the goodness of seed samples. Application of statistical methods in biological experiments is called Biometry. A very important use of biometry in Genetics is for testing whether an observed ratio may be taken as fit for an expected ratio. For examples, the expected F2 phenotypic monohybrid ratio is [...]