In this article we will discuss about the chi-square method for testing the goodness of seed samples. Application of statistical methods in biological experiments is called Biometry. A very important use of biometry in Genetics is for testing whether an observed ratio may be taken as fit for an expected ratio. For examples, the expected F2 phenotypic monohybrid ratio is [...]
The following points highlight the five main steps of hybridization technique. The steps are: 1. Selection and Preparation of Parents 2. Emasculation 3. Bagging 4. Pollination 5. Selection. Hybridization Technique # Step 1. Selection and Preparation of Parents: The plant breeder must have a clear idea about the plants which he wants to use as parents — a subject which [...]
The following points highlight the four methods of embedding plant tissues in paraffin blocks. The methods are: 1. Fixation 2. Washing 3. Dehydration 4. Infiltration. Method # 1. Fixation: (a) Flower buds: Flower buds of suitable size are rinsed in Carnoy's fluid for 5 sec. and then fixed in Nawaschin's fixative (Belling's modification) for 24 hours. Nawaschin A and B [...]