The below mentioned article provides a study notes on Single Cell Clone. By culturing a single isolated cell single cell clone can be obtained. Single cell clones derived from callus or suspension culture are heterogenous. They differ from one another in shape, texture, friability, colour, growth and morphogenetic potential etc. Working with Nicotiana tabacum L. Winsconsin ('38), Murashige and Nakamo [...]
The below mentioned article provides a quick notes on Isolation of Protoplasts and Protoplast Culture. Klerker (1892) made unsuccessful attempts to isolate protoplasts. In 1909 Kunster isolated protoplasts by mechanical method. Effective technique of protoplast isolation was evolved by Cocking ('60), Nagata and Takebe ('70) and Bhojwani and Cocking ('72). Enzymes used for isolation of protoplasts are cellulase, hemicellulase and [...]
The below mentioned article provides an overview on Protoplast Fusion and Somatic Hybridization. Plant breeders sometimes face difficulties due to sexual incompatibility. This difficulty can be removed by protoplast fusion. By this method wider crosses are possible. Even crosses between plants showing physical and chemical incompatibilities are possible by protoplast fusion. In 1909 Kunster reported random fusion between mechanically isolated [...]