According to Murashige of California University, micro-propagation is distinctly divided into five stages. Stage 0 — Mother Plant Selection: Before initiation of micro-propagation, selection of suitable mother plant is crucial in the whole exercise of propagation. Generally, disease free mother explant is selected for the micro-propagation to reduce contamination of cultures. Certain growth parameters of mother plant can be improved [...]
In this article we will discuss about: 1. Process of Somatic Embryogenesis 2. Embryo Maturation and Synchronisation 3. Cultural Conditions 4. Recurrent Embryogenesis and Mass Production 5. Applications. After fertilization, zygote is transformed into adult status through a series of embryogenic processes. Despite the same genetic constituents, somatic cells on the other hand, do not reorient towards embryo production. However, [...]
The following points highlight the three main factors affecting micro-propagation. The factors are: 1. Genotype 2. Media 3. Cultural Conditions. Factor # 1. Genotype: Physiological conditions of the donor plants influence considerably in the process of morphogenesis. Larger sizes of the meristematic tissues are advantageous. Selecting juvenile parts of the plants yield better results than older ones. The most actively [...]