The following points highlight the nine main techniques of protoplast culture. The techniques are: 1. Agar Plating Method 2. Micro-Chamber 3. Nurse Culture 4. Immobilization of Protoplast 5. Nutrient Requirement 6. Growth Regulators 7. Cultural Conditions 8. Cell Wall Regeneration 9. Regeneration. Technique # 1. Agar Plating Method: Protoplast can be cultured conveniently by using agar plating method. Two millilitres [...]
Structural organization of chloroplast is signified by the presence of double membrane envelope and soluble phase, the stroma, and an internal membrane system, the thylakoids. Both thylakoid and stromal systems are committed for light reaction and carbon dioxide fixation respectively. Chloroplast attains diversified shapes. Higher plants exhibit lens shaped chloroplasts in their cytosol. The size measures anywhere between 5 and [...]
The following points highlight the three types of hormones which regulates the growth and development of plants. The hormones are: 1. Auxins 2. Cytokinins 3. Gibberellins. Hormone # 1. Auxins: Both naturally occurring and synthetic auxins are available and are widely used in cultures for clonal propagation work. Naturally occurring auxin is indole acetic acid (IAA). Synthetic ones are 2, [...]