In this article we will discuss about:- 1. Anther Culture Technique 2. Microspore Culture 3. Diploitization 4. Anther Vs. Pollen Culture 5. Plant Regeneration 6. Characteristics of Microspore Derived Plants 7. Ontogeny of Androgenesis. The term haploid refers to those plants which contains gametophytic number of chromosomes. Anther/pollen culture mediated production of haploid plants approves the concept of totipotency. Guha [...]
In the preparation of culture media, different stock solutions (ATOG) are made. Generally stock solutions of major elements are prepared by 10X final concentration whereas minor elements and vitamins are prepared by 1000X final concentration and stored in the freezer. Stock solutions: A – NH4NO3 B – KNO3 C – CaCl2.2H2O D – KH2PO4 E — Molybdenum CaCl2 KI F [...]
The following points highlight the nine main factors affecting in vitro androgenesis. The factors are: 1. Age and Physiology 2. Stages of Pollen 3. Thermal Stress 4. Genotype 5. Chemical Treatment 6. Medium Factor 7. Anther Wall Factor 8. Microspore Density 9. Cultural Conditions. Factor # 1. Age and Physiology: The physiological conditions of the donor plant have a considerable [...]