In this article we will discuss about the process of cybrids production. These are cytoplasmic hybrids. In cybrids, nucleus of one parent and cytoplasm of both the parents constitute cytoplasmic hybrids. More precisely, cybrids can be accomplished by fusing protoplast derived from one of the parent plants with enucleated protoplast of other. Cybrids can also be obtained through various other [...]
In this article we will discuss about:- 1. Anther Culture Technique 2. Microspore Culture 3. Diploitization 4. Anther Vs. Pollen Culture 5. Plant Regeneration 6. Characteristics of Microspore Derived Plants 7. Ontogeny of Androgenesis. The term haploid refers to those plants which contains gametophytic number of chromosomes. Anther/pollen culture mediated production of haploid plants approves the concept of totipotency. Guha [...]
In the preparation of culture media, different stock solutions (ATOG) are made. Generally stock solutions of major elements are prepared by 10X final concentration whereas minor elements and vitamins are prepared by 1000X final concentration and stored in the freezer. Stock solutions: A – NH4NO3 B – KNO3 C – CaCl2.2H2O D – KH2PO4 E — Molybdenum CaCl2 KI F [...]