In this article we will discuss about the mode of action of antisense RNA. Several possible mechanisms are involved during antisense inhibition of specific gene. Following are some of the most promising mode of actions have been suggested (Fig. 22.3). Action in the Nucleus Premise: One of the earliest methods in antisense inhibition is carried out by blocking transcription. This [...]
In this article we will discuss about the concept and development of bioinformatics. Concept of Bioinformatics: Bioinformatics or life science informatics has emerged as a new branch of biotechnology, offering a fundamental tool to the biologist to accelerate commercialization of biotechnology. Bioinformatics is the classic example of convergence of biotechnology and information technolÂogy. Bioinformatics has been the most powerful tools [...]
In this article we will discuss about the manipulation of fruit softening by antisense RNA. Plant cell wall is responsible for providing mechanical strength to plant organs. Tissue and cells due to their complex structure and biomolecules are involved. During fruit ripening several hydrolytic enzymes such as PG, PME and cellulase cleaves various polymers that conÂstitute cell wall and consequently [...]