In this article we will discuss about the tests for estimation of ascorbic acid in plants. Principle: Ascorbic acid oxidase acts on the substrate ascorbic acid to form dehydro-ascorbic acid. The residual ascorbic acid content is determined by titration using a dye solution (Dichlorophenol indophenol, an oxidation-reduction dye): Requirements: (a) Reagents: 1. Dye solution: 15 mgm of 2-6 dichlorophenol indophenol [...]
In this article we will discuss about the tests for detection of tannins in plants. Tannins are a heterogeneous group of organic compounds found in plants. They are commonly present in unripe fruits — guava, banana and leaves of tea etc. These are water-soluble compounds. The metabolic significance of these compounds are not clearly known but they play a significant [...]
A number of inorganic elements are present in plants which also catalyse many enzymatic reactions. The existence of such inorganic elements in plants can be detected by the chemical tests of plant ash. Requirements for Detection of Ash: (a) Preparation of Ash Extract: For detection of various elements in plant ash viz., Ca, Mg, Fe, S, P, CI etc. ash [...]