In this article we will discuss about the tests for estimation of ascorbic acid in plants. Principle: Ascorbic acid oxidase acts on the substrate ascorbic acid to form dehydro-ascorbic acid. The residual ascorbic acid content is determined by titration using a dye solution (Dichlorophenol indophenol, an oxidation-reduction dye): Requirements: (a) Reagents: 1. Dye solution: 15 mgm of 2-6 dichlorophenol indophenol [...]
In this article we will discuss about the tests for detection of tannins in plants. Tannins are a heterogeneous group of organic compounds found in plants. They are commonly present in unripe fruits — guava, banana and leaves of tea etc. These are water-soluble compounds. The metabolic significance of these compounds are not clearly known but they play a significant [...]
In this article we will discuss about the tests for detection of fats and oils in plants with the help of experiments. Fats and Oils are invariably present in all living cells. They are synthesized through an active metabolism and also serve a number of indispensable roles in physiological processes. Fats are the esters of fatty acids with glycerol and [...]