In this article we will discuss about the tests for detection of fats and oils in plants with the help of experiments. Fats and Oils are invariably present in all living cells. They are synthesized through an active metabolism and also serve a number of indispensable roles in physiological processes. Fats are the esters of fatty acids with glycerol and [...]
In this article we will discuss about the tests for estimation of amylase in plants. Principle: The reducing sugars produced by the action of α- and or β-amylase react with di-nitro-salicylic acid and reduce it to a brown coloured product, nitro-amino-salicylic acid. Materials: i. Sodium acetate buffer, 0.1 M pH 4.7 ii. Starch 1% Solution: Prepare a fresh solution by [...]
A number of inorganic elements are present in plants which also catalyse many enzymatic reactions. The existence of such inorganic elements in plants can be detected by the chemical tests of plant ash. Requirements for Detection of Ash: (a) Preparation of Ash Extract: For detection of various elements in plant ash viz., Ca, Mg, Fe, S, P, CI etc. ash [...]